Another aspect of this practical that was evaluated is the within-run precision, also known as intra-assay precision and the between-run precision or inter-assay precision, as it is also referred to as. Within-run precision is used to determine the precision of the assay being used. This is carried out by measuring the absorbance of a pooled plasma sample a number of times, assessing the repeatability of the results and so, the precision of the results. The results are assessed on how close they are in agreement to each other after being measured successively under the same conditions. In this practical the sample was measured 10 times. The ideal amount of times a sample should be repeated at a given level, in a single day and in a single run is 20. This will give an idea of the repeatability of the assay. The mean, standard deviation and percentage coefficient of variation were all obtained in order to determine how precise the assay was. Between-run precision was also evaluated. This is a better indicator of the precision of a method than that of within run. This is so as it is a measure of random error of the method. One of these errors being different people carrying out the assay. The EP05-A2, a document produced by the CLSI, is intended to determine the precision of the methods used by the laboratory. This assessment is carried out at two levels, containing at least ten samples per run and should contain at least one sample of quality control (Chesher, 2008). The class results for the albumin concentration (g/l) for both Control 1 and Control 3 were used for this evaluation. Again, the mean, standard deviation and percentage coefficient of variation were all determined and compared to that of the manufacturers. This enabled the comparison of the class results for each control and the manufacturer’s control results in terms of precision .
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